Dawn-to-dusk dry fasting induces anti-atherosclerotic, anti-inflammatory, and anti-tumorigenic proteome in peripheral blood mononuclear cells in subjects with metabolic syndrome
Abstract
Background: Metabolic syndrome characterized by abdominal obesity, high blood pressure, elevated fasting glucose and triglyceride levels and low high-density lipoprotein cholesterol level is associated with pro-inflammatory state, increased risk for atherosclerosis, and multiple cancers. Our previous results on subjects with metabolic syndrome showed that 4-week dawn-to-dusk (sunset) dry fasting resulted in significant changes in the serum proteome and improvement in several metabolic risk factors. Peripheral blood mononuclear cells (PBMC) proteomics is a powerful tool that can provide mechanistic insights into how dawn-to-dusk dry fasting affects protein expression in metabolic pathways at cellular level. In this study, we determined whether dawn-to-dusk dry fasting would induce favorable changes in PBMC proteome in subjects with metabolic syndrome, similar to the changes induced by dawn-to-dusk dry fasting in the same subjects' serum proteome.
Background: Metabolic syndrome characterized by abdominal obesity, high blood pressure, elevated fasting glucose and triglyceride levels and low high-density lipoprotein cholesterol level is associated with pro-inflammatory state, increased risk for atherosclerosis, and multiple cancers. Our previous results on subjects with metabolic syndrome showed that 4-week dawn-to-dusk (sunset) dry fasting resulted in significant changes in the serum proteome and improvement in several metabolic risk factors. Peripheral blood mononuclear cells (PBMC) proteomics is a powerful tool that can provide mechanistic insights into how dawn-to-dusk dry fasting affects protein expression in metabolic pathways at cellular level. In this study, we determined whether dawn-to-dusk dry fasting would induce favorable changes in PBMC proteome in subjects with metabolic syndrome, similar to the changes induced by dawn-to-dusk dry fasting in the same subjects' serum proteome.
Anthropometric, hemodynamic, metabolic, and renal responses during 5 days of food and water deprivation
https://www.ncbi.nlm.nih.gov/pubmed/24434757
Abstract
Background: Although there is considerable research in the field of fasting and fluid restriction, little is known about the impact of food and water deprivation (FWD) on body circumferences and vital parameters.
Methods: During 5 days of FWD in 10 healthy adults, hemodynamic, metabolic, and renal parameters, such as weight, 5 circumferences at neck, waist, hip, chest at axilla, chest at nipples, and 1 new oblique hip circumference were measured daily. For each circumference, new quotients of daily circumference-to-weight decrease were calculated. The set of employed parameters quantified and monitored dieting persons' compliance and efficacy of the method.
Results: The values of blood pressure, heart rate, hemoglobin oxygen saturation, glucose, K(+), Na(+), Cl(-), urea, creatinine, and serum osmolality proved to be stable. The mean creatinine clearance increased up to 167%. The mean daily weight decrease (1,390 ± 60 g) demonstrated the effectiveness of FWD in weight reduction. The daily decrease of all measured circumferences and the values of the corresponding circumference-to-weight decrease quotients reflected considerable volume decrease in all measured body parts per day and kg of weight loss during FWD.
Conclusion: The intervention of 5 FWD days in 10 healthy adults was found to be safe, decreased weight and all measured circumferences, and improved renal function considerably.
Abstract
Background: Although there is considerable research in the field of fasting and fluid restriction, little is known about the impact of food and water deprivation (FWD) on body circumferences and vital parameters.
Methods: During 5 days of FWD in 10 healthy adults, hemodynamic, metabolic, and renal parameters, such as weight, 5 circumferences at neck, waist, hip, chest at axilla, chest at nipples, and 1 new oblique hip circumference were measured daily. For each circumference, new quotients of daily circumference-to-weight decrease were calculated. The set of employed parameters quantified and monitored dieting persons' compliance and efficacy of the method.
Results: The values of blood pressure, heart rate, hemoglobin oxygen saturation, glucose, K(+), Na(+), Cl(-), urea, creatinine, and serum osmolality proved to be stable. The mean creatinine clearance increased up to 167%. The mean daily weight decrease (1,390 ± 60 g) demonstrated the effectiveness of FWD in weight reduction. The daily decrease of all measured circumferences and the values of the corresponding circumference-to-weight decrease quotients reflected considerable volume decrease in all measured body parts per day and kg of weight loss during FWD.
Conclusion: The intervention of 5 FWD days in 10 healthy adults was found to be safe, decreased weight and all measured circumferences, and improved renal function considerably.
Dry Fasting Physiology: Responses to Hypovolemia and Hypertonicity
https://pubmed.ncbi.nlm.nih.gov/31958788/
Objective: The aim of this study was to provide a deeper insight into dry fasting (DF) physiology.
Design: Ten participants performed DF for 5 consecutive days.
Methods: The following parameters were monitored daily: cortisol, aldosterone, high-sensitivity C-reactive protein (CRP), erythropoietin, albumin, uric acid, and vitamin C in serum; vasopressin (ADH), adrenocorticotropic hormone (ACTH), renin, angiotensin II, and total antioxidant capacity (TAC) in plasma; hematocrit and erythrocytes in whole blood; osmolality, noradrenaline, dopamine, adrenaline, Na+, and K+ in 24-h urine; waist circumference and body, urine, and stool weight.
Results: The following parameters increased: ADH (60 ± 11%), ACTH (176 ± 34%), cortisol (495 ± 75%), urine osmolality (20 ± 4%), CRP (167 ± 77%), renin (315 ± 63%), angiotensin II (74 ± 21%), aldosterone (61 ± 21%), TAC (80.4 ± 17%), uric acid (103 ± 19%), albumin (18.4 ± 2.4%), erythrocytes (13.4 ± 2.2%), hematocrit (11 ± 1.8%), and the excretion of noradrenaline (40.3 ± 10%) and dopamine (17 ± 5%). The following parameters decreased: waist circumference (8.20 ± 0.61 cm), body weight (7.010 ± 0.3 kg), erythropoietin (65 ± 18%), and the excretion of adrenaline (38 ± 4%) and Na+ (60 ± 16%). The excretion of K+ remained unchanged. Vitamin C decreased, showing a half-life of 4.8 ± 0.7 days. The percent ratios of lost weight components were: urine (52.2 ± 3.7%), insensible water loss (32.2 ± 1.4%), stool (5 ± 0.3%), and respiratory gases, i.e., expired CO2 - incorporated O2 (10.6 ± 5.4%).
Conclusion: The mechanisms underlying the hypertonicity and hypovolemia compensation and the ratio analysis of lost weight components were presented. DF demonstrated short-term antioxidant, anti-ischemic, immune-stimulating, anti-edematous, and anti-inflammatory effects. The results may have an impact on developing new concepts for the treatment of edema, obesity, and inflammatory and ischemic diseases.
Keywords: Anti-edematous effect; Anti-inflammatory effect; Anti-ischemic effect; Hypertonicity and hypovolemia compensation mechanisms; Immune-stimulating effect; Ratio analysis of lost weight components; Vitamin C half-life time.
Dr. Chennikov carried out a clinical study in 1992 validated by the government of Russia. The empirical results cannot be refuted. In the Russian Federation, Dr. Chennikov was the first to obtain a patent for such an unusual invention, and at that time considered almost unscientific. patent No. 2028160 for invention "A method of re-education of the body" (Technique of abstinence from water and food to heal) The purpose of the study: To increase the therapeutic effect of therapeutic fasting, creating a comprehensive and highly effective methodology for combating various diseases for use in clinical and private conditions. Dry Fasting Method: Detoxify the body of these wastes, restore the body's immunological status, increase the body's resistance to external influences (law of hormesis), prevent and eliminate physical and psychological disorders of the human body. The method, hereinafter referred to as the "abstinence cure method", was officially tested in 1992 on a group of 19 people, including a member of the expert commission, in accordance with an agreement concluded with the Institute of Ivanovo State Medicine (IGMI), the Enterprise for the Research and Production of Environmental Medicines, Oncological Protection, Ivanov Gastroenterological Sanatorium in Russia. The group included people between the ages of 20 and 63 with hypertension, osteochondrosis, cardiovascular disease, lung cancer, bronchial asthma, kidney stones, digestive disorders, gastric ulcers, varicose veins, metabolic disorders, chronic ENT disorders. Various autoimmune diseases, uterine fibroids. The approval took place in sanatorium conditions and was accompanied by a competent commission of experts consisting of: Chernobrovy V.F. - Vice-Rector for Medical Works of IGMI, Head of the Department of Infectious Diseases, Professor; Bobkov V.A. - head of the department of internal medicine, professor; Poltyrev V.S. - Head of the Department of Internal Medicine, Faculty of Higher Medical Studies, Professor; Nikolayenkov Yu.V. - Vice-rector for academic affairs, IGMI, head of the physiopathology department, professor; Citizen L.S. - head of the department of normal physiology, professor; Slobodin V.B. - Head of the Department of Biochemistry, Professor. Experimental testing of L. A. Chennikov's method of abstinence healing was accompanied by extensive clinical evaluation and monitoring of biochemical, immunological and physiological (blood drawing) parameters. The dynamics of blood, urine, blood pressure, changes in body weight, etc., were taken into account by the expert commission, which reviewed the clinical, laboratory and functional documentation for the tests clinics of the method of young dry taking into account the dynamics of changes in indicators, phases of resistance, types and nature of cleansing the body of toxins, etc. After hearing the report of the author of the method "Healing by abstinence from water and solid", L. A. Chennikov, as well as the results of the clinical experience obtained to evaluate this method, presented by the associate professor of 'IGMI, E. V. Putintsev, who was among the participants in the experiment. Clinical, laboratory and functional studies and a survey of patients who underwent a two-week course of fasting dry. (including 2-day preparation and 3-day outing), concluded: From 12.10 to 26.10.92, Associate Professor E.V. Putintsev attended a course of Healing Abstinence according to the method of L. A. Chennikov Consisting of 20 people and the leader of a scientific experiment. attached) on the basis of the Ivanovo children's gastroenterological sanatorium - the chief physician V. Bunin The expert commission confirms the reliability of all clinical, laboratory and functional documentation of the "Healing Abstinence" clinical experience. The expert commission confirms all the results of the scientific experiment "Healing water and solid abstinence": dynamics of clinical symptoms, types and nature of symptoms of cleansing the body of toxins and diseased tissues, changing resistance phases , energy dynamics, vascular and laboratory parameters. The expert committee confirms the positive results expressed in 18 out of 20 patients, with an improvement in blood counts The expert commission recommends the use of the method of "healing by abstinence from water and solids" for therapeutic purposes for a wide range of pathologies. Evaluates the young dry method to be 96% reliable on all pathologies combined.
Objective: The aim of this study was to provide a deeper insight into dry fasting (DF) physiology.
Design: Ten participants performed DF for 5 consecutive days.
Methods: The following parameters were monitored daily: cortisol, aldosterone, high-sensitivity C-reactive protein (CRP), erythropoietin, albumin, uric acid, and vitamin C in serum; vasopressin (ADH), adrenocorticotropic hormone (ACTH), renin, angiotensin II, and total antioxidant capacity (TAC) in plasma; hematocrit and erythrocytes in whole blood; osmolality, noradrenaline, dopamine, adrenaline, Na+, and K+ in 24-h urine; waist circumference and body, urine, and stool weight.
Results: The following parameters increased: ADH (60 ± 11%), ACTH (176 ± 34%), cortisol (495 ± 75%), urine osmolality (20 ± 4%), CRP (167 ± 77%), renin (315 ± 63%), angiotensin II (74 ± 21%), aldosterone (61 ± 21%), TAC (80.4 ± 17%), uric acid (103 ± 19%), albumin (18.4 ± 2.4%), erythrocytes (13.4 ± 2.2%), hematocrit (11 ± 1.8%), and the excretion of noradrenaline (40.3 ± 10%) and dopamine (17 ± 5%). The following parameters decreased: waist circumference (8.20 ± 0.61 cm), body weight (7.010 ± 0.3 kg), erythropoietin (65 ± 18%), and the excretion of adrenaline (38 ± 4%) and Na+ (60 ± 16%). The excretion of K+ remained unchanged. Vitamin C decreased, showing a half-life of 4.8 ± 0.7 days. The percent ratios of lost weight components were: urine (52.2 ± 3.7%), insensible water loss (32.2 ± 1.4%), stool (5 ± 0.3%), and respiratory gases, i.e., expired CO2 - incorporated O2 (10.6 ± 5.4%).
Conclusion: The mechanisms underlying the hypertonicity and hypovolemia compensation and the ratio analysis of lost weight components were presented. DF demonstrated short-term antioxidant, anti-ischemic, immune-stimulating, anti-edematous, and anti-inflammatory effects. The results may have an impact on developing new concepts for the treatment of edema, obesity, and inflammatory and ischemic diseases.
Keywords: Anti-edematous effect; Anti-inflammatory effect; Anti-ischemic effect; Hypertonicity and hypovolemia compensation mechanisms; Immune-stimulating effect; Ratio analysis of lost weight components; Vitamin C half-life time.
Dr. Chennikov carried out a clinical study in 1992 validated by the government of Russia. The empirical results cannot be refuted. In the Russian Federation, Dr. Chennikov was the first to obtain a patent for such an unusual invention, and at that time considered almost unscientific. patent No. 2028160 for invention "A method of re-education of the body" (Technique of abstinence from water and food to heal) The purpose of the study: To increase the therapeutic effect of therapeutic fasting, creating a comprehensive and highly effective methodology for combating various diseases for use in clinical and private conditions. Dry Fasting Method: Detoxify the body of these wastes, restore the body's immunological status, increase the body's resistance to external influences (law of hormesis), prevent and eliminate physical and psychological disorders of the human body. The method, hereinafter referred to as the "abstinence cure method", was officially tested in 1992 on a group of 19 people, including a member of the expert commission, in accordance with an agreement concluded with the Institute of Ivanovo State Medicine (IGMI), the Enterprise for the Research and Production of Environmental Medicines, Oncological Protection, Ivanov Gastroenterological Sanatorium in Russia. The group included people between the ages of 20 and 63 with hypertension, osteochondrosis, cardiovascular disease, lung cancer, bronchial asthma, kidney stones, digestive disorders, gastric ulcers, varicose veins, metabolic disorders, chronic ENT disorders. Various autoimmune diseases, uterine fibroids. The approval took place in sanatorium conditions and was accompanied by a competent commission of experts consisting of: Chernobrovy V.F. - Vice-Rector for Medical Works of IGMI, Head of the Department of Infectious Diseases, Professor; Bobkov V.A. - head of the department of internal medicine, professor; Poltyrev V.S. - Head of the Department of Internal Medicine, Faculty of Higher Medical Studies, Professor; Nikolayenkov Yu.V. - Vice-rector for academic affairs, IGMI, head of the physiopathology department, professor; Citizen L.S. - head of the department of normal physiology, professor; Slobodin V.B. - Head of the Department of Biochemistry, Professor. Experimental testing of L. A. Chennikov's method of abstinence healing was accompanied by extensive clinical evaluation and monitoring of biochemical, immunological and physiological (blood drawing) parameters. The dynamics of blood, urine, blood pressure, changes in body weight, etc., were taken into account by the expert commission, which reviewed the clinical, laboratory and functional documentation for the tests clinics of the method of young dry taking into account the dynamics of changes in indicators, phases of resistance, types and nature of cleansing the body of toxins, etc. After hearing the report of the author of the method "Healing by abstinence from water and solid", L. A. Chennikov, as well as the results of the clinical experience obtained to evaluate this method, presented by the associate professor of 'IGMI, E. V. Putintsev, who was among the participants in the experiment. Clinical, laboratory and functional studies and a survey of patients who underwent a two-week course of fasting dry. (including 2-day preparation and 3-day outing), concluded: From 12.10 to 26.10.92, Associate Professor E.V. Putintsev attended a course of Healing Abstinence according to the method of L. A. Chennikov Consisting of 20 people and the leader of a scientific experiment. attached) on the basis of the Ivanovo children's gastroenterological sanatorium - the chief physician V. Bunin The expert commission confirms the reliability of all clinical, laboratory and functional documentation of the "Healing Abstinence" clinical experience. The expert commission confirms all the results of the scientific experiment "Healing water and solid abstinence": dynamics of clinical symptoms, types and nature of symptoms of cleansing the body of toxins and diseased tissues, changing resistance phases , energy dynamics, vascular and laboratory parameters. The expert committee confirms the positive results expressed in 18 out of 20 patients, with an improvement in blood counts The expert commission recommends the use of the method of "healing by abstinence from water and solids" for therapeutic purposes for a wide range of pathologies. Evaluates the young dry method to be 96% reliable on all pathologies combined.